ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 75 kDa band observed in purified membranes of rat insulinoma cell line RIN14B (calculated MW of 48.4 kDa according to NP_033957.1). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51 (11) :1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows strong staining in islets of Langerhans and weak staining in acinar cells. Recommended concentration, 3 μg/mL.
CCKBR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CCKBR antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Rat