ELISA: Antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments in human pancreas lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). Immunohistochemistry: In paraffin embedded human pancreas, shows cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10 μg/mL.
CCKAR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CCKAR antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Rat