ELISA: Antibody detection limit dilution 1:64,000. Approximately 75 kDa band observed in purified membranes of rat insulinoma cell line RIN14B (calculated MW of 48.4 kDa according to NP_033957.1). Data kindly provided by Dr. Morisset, Sherbrooke, Canada. Data accepted by International Journal of Endocrinology (in press). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51 (11) :1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3 μg/mL.
CCKA Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CCKA Receptor antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.