ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 22 kDa band observed in lysates of human fibroblast cell lines A431 and HeLa (calculated MW of 22.2 kDa according to NP_036249.1). Recommended concentration: 0.03-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human uterus shows strong nuclear staining in the glandular epithelial cells. Recommended concentration, 2-3 μg/mL.
CBX5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CBX5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.