ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 25 kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25 kDa according to NP_006798.1). Recommended concentration: 0.3-1.0 μg/mL. Immunohistochemistry: In paraffin embedded human placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3 μg/mL.
CBX1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CBX1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.