ELISA: Antibody detection limit dilution >1:32,000. Approximately 35 kDa band observed in HEK293 cell lysate (calculated MW of 30.9 kDa according to NP_001227.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bains et al, J Pharmacol Exp Ther. 2010 Mar;332(3):755-63.; PMID: 20007405). Recommended concentration: 1-3 μg/mL.
CBR3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CBR3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.