ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 30-35 kDa band seen in human liver and kidney lysates [Calculated MW of 30.4 kDa according to NP_001748.1]. Recommended for use at 0.2-0.5 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration, 1-2 μg/mL.
CBR1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CBR1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.