ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 65 kDa band observed in rat brain lysates (calculated MW of 52.7 kDa according to rat NP_036916.1 and 52.9 kDa according to human NP_057167.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 10762343). Recommended concentration: 0.1-0.3 μg/mL.
Cannabinoid Receptor 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cannabinoid Receptor 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.