ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 70 kDa band observed in human cerebellum lysates (calculated MW of 42.8 kDa according to NP_660339.1). The observed molecular weight corresponds to findings with EB08040, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.03-0.1 μg/mL.
CADM4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CADM4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.