ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 55 kDa band observed in human heart lysates (calculated MW of 52.4 kDa according to NP_068757.2). Recommended concentration: 1-3 μg/mL.
Bruno-like 5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Bruno-like 5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.