ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 45-48 kDa band observed in human brain lysates (calculated MW of 42.6 kDa according to NP_001814.2). Recommended concentration: 0.1-0.3 μg/mL.
Brain Creatine Kinase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Brain Creatine Kinase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.