ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 35 kDa band observed in lysates of cell lines Daudi, K562 and U937 calculated MW of 40.5 kDa according to NP_000701.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 2-4 μg/mL.
Bradykinin Receptor B1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Bradykinin Receptor B1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.