ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 22 kDa band in human testis lysates after 1.5 μg/mL antibody staining.6 kDa according to NP_932074.1 and 31.3 kDa according to NP_149019.1. The 22 kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36 kDa band in human and mouse testis (PMID: 11390979 ).
BOULE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
BOULE antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.