ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 28 kDa band observed in human kidney lysates (calculated MW of 26.1 kDa according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1). Recommended concentration: 0.5-1.5 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa . This band was successfully blocked by incubation with the immunizing peptide.
BNIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
BNIP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.