ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 38-40 kDa band observed in rat skin lysates (calculated MW of 41.7 kDa according to rat NP_058783.1 and human NP_001702.1 ). Recommended concentration: 0.1-0.3 μg/mL.
Biglycan Preproprotein antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Biglycan Preproprotein antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.