ELISA: Antibody detection limit dilution 1:64,000. Approximately 55 + 48 kDa bands observed in nuclear HeLa cell lysate (calculated MW of 56.0 kDa according to NP_001015049.1 and of 51.2 kDa according to NP_004864.1; NP_001015048.1). Recommended concentration: 1-3 μg/mL.
BAG5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
BAG5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.