ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 18 kDa band observed in mouse brain lysates (calculated MW of 15.6 kDa according to human NP_079088.1 and of 15.5 kDa according to mouse NP_542371.1. Recommended concentration: 0.03-0.1 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa in both human and mouse brain lysates. This band was successfully blocked by incubation with the immunizing peptide.
BAALC antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
BAALC antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse