ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 38-40 kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5 kDa according to NP_006351.2). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10 μg/mL.
B5 Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
B5 Receptor antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.