ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 170 kDa band observed in human brain (Substantia Nigra) lysates (calculated MW of 47.0 kDa according to NP_000698.1). The observed molecular weight corresponds to findings with EB08786, a product of different design reacting to the mouse protein. Recommended concentration: 0.05-0.2 μg/mL. Immunohistochemistry: In paraffin embedded human Pituitary shows bright cytoplasm staining of a few active secretory cells in the anterior pituitary. Recommended concentration, 5-10 μg/mL.
AVPR1B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
AVPR1B antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.