ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 35 kDa band seen in HeLa cell lysates. Recommended for use at 0.5-2 μg/mL. Please note the band size is in agreement with previous experimental data (J Biol Chem 2001 Apr 27;276 (17) :13718-26). Immunohistochemistry: In paraffin embedded human testis shows staining of select spermatocytes in human testis. Recommended concentration, 3-5 μg/mL.
ATF7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ATF7 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.