ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 40 kDa band observed in human brain lysates (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows mainly nuclear staining of Purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6 μg/mL.
Arrestin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Arrestin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat