ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 50-55 kDa band observed in human placenta lysates (calculated MW of 57.9 kDa according to NP_000094.2 and NP_112503.1). Recommended concentration: 0.1-0.3 μg/mL.
Aromatase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Aromatase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.