ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 80 kDa band observed in human brain lysates (calculated MW of 79.1 kDa according to NP_056135 and 76.4 kDa according to 127462). Recommended concentration: 2-4 μg/mL.
ARHGEF4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ARHGEF4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.