ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 18 kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7 kDa according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). Recommended concentration: 1-3 μg/mL.
ARF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ARF1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.