ELISA: Antibody detection limit dilution 1:2,000. Western Blot: 30 kDa band observed in A431 and human lymph node lysates (predicted MW 29 kDa according to NP_006392). Recommended for use at 0.2-1 μg/mL.
APRIL antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
APRIL antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.