ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 80 kDa band observed in rat Aortic Smooth muscle Cells (lane 1), intensity increased after infection with human aPPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7 kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1 μg/mL.
APPL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
APPL1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.