ELISA: Antibody detection limit dilution 1:128,000. Approximately 100 kDa band observed in lysates of NIH/3T3 and HeLa (calculated MW of 77.2 kDa according to NP_001155.1 and 77.0 kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273 (32) :20128-33.; PMID: 9685356). Recommended concentration: 0.1-0.3 μg/mL.
APBB1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
APBB1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse