ELISA: Antibody detection limit dilution 1:8000. Western Blot: At 1 μg/mL antibody banded at approximately 45-48 kDa in Hela, A431 and human kidney lysates. Immunohistochemistry: In paraffin embedded human pancreas shows staining of islet of Langerhans. Recommended concentration, 3-5 μg/mL.
Annexin A10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Annexin A10 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.