ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 35 kDa band observed in human heart, liver and muscle lysates (calculated MW of 47.0 kDa according to NP_061041.2 (alpha isoform) and 31.2 kDa according to AAF71042.1 (beta isoform). In transfected HEK293 transiently expressing ALS2CR3 a band of approximately 48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration 1-3 μg/mL. Immunohistochemistry: Immunohistochemistry: In paraffin embedded human heart shows staining of myocardial fibres in transverse section. Recommended concentration, 3-5 μg/mL.
ALS2CR2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ALS2CR2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.