ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 48 kDa band in human brain and U937 lysates at 1 μg/mL. Further, this band was successfully blocked by incubation with the immunizing peptide. ALOX5AP antibody has been successfully used in WB assays with transfected HeLa cells (customer feedback, see Maier TJ et al., 2008).
ALOX5AP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ALOX5AP antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.