ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 35 kDa band observed in human liver lysates (calculated MW of 37.1 kDa according to NP_001809.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4 μg/mL.
AKR1C4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
AKR1C4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.