ELISA: Antibody detection limit dilution 1:64,000. Western Blot: experiments gave bands at approximately 80-90 kDa and strong ladder from 26 to 38 kDa in human prostate lysates after 0.3 μg/mL antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature with different antibodies (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul;31 (1) :13-21; PMID: 14742294). This protein has a calculated MW of 87.7 kDa according to NP_079496.1. Recommended concentration: 0.1-0.3 μg/mL.
ADAM33 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ADAM33 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.