ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 60 kDa band observed in human Umbilical Cord lysates (calculated MW of 57.2 kDa according to NP_001096.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows preferential staining of cytoplasm in primary spermatocytes. Recommended concentration, 4-6 μg/mL.
ACVR1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ACVR1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.