ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 45 kDa band observed in rat testis lysates (calculated MW of 45.5 kDa according to rat NP_001020588.1 and 45.6 kDa according to mouse NP_079547.1). Recommended concentration: 0.5-1.5 μg/mL. an additional band of unknown identity was also consistently observed at 30 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Actin-like 7B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Actin-like 7B antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.