ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 75 kDa band observed in human liver lysates (calculated MW of 76.8 kDa according to NP_003491.1). Recommended concentration: 0.5-1.5 μg/mL. an additional band of 37 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
ACOX2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ACOX2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.