ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 48 kDa band in human brain (cerebellum, cerebral cortex and frontal cortex) lysates after 1 μg/mL antibody staining. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.
ABHD4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ABHD4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.