ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 170 kDa band observed in rat renal cortex membranes (calculated MW of 149 kDa according to rat NP_596902.1 and 150 kDa according to human NP_005836.2). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4 μg/mL.
ABCC4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ABCC4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Rat