ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 28 kDa band observed in human skin lysates (calculated MW of 27.8 kDa according to NP_006133.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-3 μg/mL.
14-3-3 sigma antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
14-3-3 sigma antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.