ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 80-85 kDa band observed in lysates of HeLa (calculated MW of 82.7 kDa according to NP_066964.1). Recommended concentration: 0.1-0.3 μg/mL.
XRCC5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
XRCC5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.