ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 35 kDa band observed in lysates of human testis and ovary (calculated MW of 39.1 kDa according to NP_110388.2). Recommended concentration: 0.03-0.1 μg/mL.
Wnt4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Wnt4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.