ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 50 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0 kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approximately 38 kDa to 45 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
TFPI antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TFPI antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.