ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 30 kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60 kDa glycoprotein (calculated MW of 33.8 kDa according to NP_000573.1). Recommended concentration: 1-3 μg/mL.
Osteopontin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Osteopontin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.