ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 28+30 kDa bands observed in human liver lysates (calculated MW of 31.1 kDa and 34.6 kDa according to NP_003022.3 and NP_001006611.1). Recommended concentration: 0.1-0.3 μg/mL.
SIAH1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SIAH1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Rat