ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 45 kDa band observed in human duodenum, liver and lung lysates (calculated MW of 45.1 kDa according to NP_000593.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human placenta shows strong staining of cytotrophoblasts. Recommended concentration, 4-6 μg/mL. Also been successfully stained paraffin embedded human breast carcinoma tissue. Enzyme Immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3 μg/mL.
PAI-1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PAI-1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.