ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 16 kDa band observed in Murine NIH 3T3 lysates (calculated MW of 15.9 kDa according to NP_002890.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas shows vesicular staining in the acinar cytoplasm but oriented towards the interlobular space. Recommended concentration, 5-10 μg/mL.
RBP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RBP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse