ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 70 kDa band observed in human brain and 3T3 lysates (predicted MW of 68 kDa according to NP_002874). Recommended for use at 0.1-0.5 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4 μg/mL.
RANGAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RANGAP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse