ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 20 kDa band observed in lysates of human tonsil lysate (calculated MW of 21.4 kDa according to NP_002863.1). Recommended concentration: 0.1-1 μg/mL. Immunohistochemistry: In paraffin embedded human tonsil, stains most cells in the mantle zone and interfollicular area. In the germinal centres the majority of cells are negative. Recommended concentration 0.3 μg/mL. IHC data were obtained with a previous batch.
RAC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RAC2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.