ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 70 kDa band observed in HeLa lysate (calculated MW of 68 kDa according to NP_055118). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows nucleolar staining in spermatocytes and spermatids. Recommended concentration, 2.5-3.8 μg/mL.
Pescadillo antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pescadillo antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.