ELISA: Antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approximately 50 kDa and 37 kDa in human brain lysate after 1 μg/mL antibody staining. Calculated MW is 50 kDa according to NP_065855.1. Both bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3 μg/mL.
PACSIN1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PACSIN1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.