ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 75 kDa band observed in HeLa lysates (calculated MW of 75 kDa according to NP_079120). Recommended concentration: 1-3 μg/mL.
NUP85 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NUP85 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.